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Easytaq buffer

WebApr 4, 2016 · The PCR amplification was performed in 20 µL of reaction mixture: 1 µL genomic DNA, 0.5 µL of each of the 4 primers (10 µmol/L), 2 µL of dNTPs (2.5 mmol/L), 2 µL of 10× EasyTaq buffer (Mg 2+ plus), 1 µL of EasyTaq polymerase (5 U/µL, TransGen), and 12 µL of ddH 2 O. The thermal profile was the following: an initial denaturation step ... WebMar 28, 2024 · EasyTaq ® PCR SuperMix for PAGE is a ready-to-use mixture of EasyTaq ® DNA Polymerase for PAGE, dNTPs and optimized buffer. The SuperMix for PAGE is …

EasyTaq® DNA Polymerase

WebMar 21, 2024 · The error-prone PCR reaction mixture contained 10 × EasyTaq buffer (2.5 μL), ddH 2 O (16.0 μL), dNTPs (2.5 mM, 2 μL), Mn 2+ (1 mM, 2.5 μL), template plasmid (0.5 μL), forward and reverse primers (10 μM, 0.5 μL each), and EasyTaq DNA polymerase (2.5 U). The PCR program was 3 min denaturation at 94 ºC, followed by 30 cycles of 95 ºC for ... WebEasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. line heater sizing https://uslwoodhouse.com

Developing a multiplex PCR‐based assay kit for bloodstream …

WebEasyTaq buffer (20 mM TrisHCl, pH 8.0, 200 mM KCl, 100 mM (NH 4) 2 SO 4, 20 mM MgSO 4, 0.6 µL of each forward and reverse primer (10 µM), 0.35 µL of 5 U/µL EasyTaq DNA polymerase). PCR amplifica tion was performed on thermal cycler (BioRad S1000, United States) using with the following condi tions: initial denaturation for 5 min at 94°C ... WebOct 14, 2024 · dNTPs、Easytaq®DNA polymerase for PAGE等试剂均来自北京全式金生物技术(TransGen Biotech)有限公司。 ... SSR-PCR总反应体系为15 μL，其中10 × PCR buffer for page 1.5 μL、0.2 mmoL·L-1dNTPs 2 μL、0.5 μmoL·L-1的正反引物各0.5 μL、DNA 模板1 μL、1 UTaqDNA聚合酶0.2 μL。 WebAug 19, 2024 · The 20 μl PCR reaction mixture contained 10× EasyTaq buffer (2 μL), 2.5 mM dNTPs (1.6 μl), 5 U/μl EasyTaq DNA Polymerase (0.2 μl), 20 μM primers (1 μL each), crude DNA template (1 μl), and sterile distilled water. Amplification was performed on a thermal cycler with a cycling profile of initial denaturation at 95°C for 5 min; 30 ... line height 1.2 in px

Development of a Microsatellite Set for Paternity Assignment …

Category:TransStart® FastPfu DNA Polymerase ABIN5519527

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Easytaq buffer

鸭坦布苏病毒的分离、鉴定及致病性研究-现代畜牧网-cvonet.com

Web- TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. - PCR products can be directly cloned into pEASY®-Blunt vectors. ... Buffer Storage Buffer: 50 mM Tris-HCl ( pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50 % glycerol 5xTransStart® FastPfu Buffer with 20 mM MgSO4: 100 mM Tris-SO4 ( pH … WebEasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE. • Extension rate is about 1-2 kb/min. • Template-independent “A” …

Easytaq buffer

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WebThe 10x EasyTaq buffer is used with EasyTaq DNA Polymerase. Additional information. Supplier: Transgen Biotech. Format: 1.2 ml. Ask a question about 10x EasyTaq Buffer (with Mg2+) – GK101. Name. Email Address. Competitor product (if applicable) Question. Get an answer quickly. Web豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座,心理学等数亿实用 ...

WebNov 16, 2024 · We performed the PCR experiment using 20 μl reaction mixtures containing 100 ng genomic DNA, 2 μl 10× EasyTaq Buffer (with Mg 2+), 1.5 μl 2.5 mM dNTPs, 2 μl each of 2 μM primer stock, and 1 U EasyTaq DNA Polymerase (TransGen Biotech, Beijing, China) under the following thermal cycler conditions: 95°C for 5 min, then 35 cycles at … WebEasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous …

WebOct 1, 2016 · EasyTaq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. This exceptionally performant Taq DNA … WebSep 19, 2024 · To determine the optimal pH for the acetophenone asymmetric bioreduction system using M190V, a range of buffers was used, with pH varying from 5.5 to 10.5. As illustrated in Figure 4c, when the buffer pH increased from 5.5 to 6, the conversion of acetophenone was obviously improved, and relatively high conversions (24.38–34.79%) …

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WebTaKaRa Taq DNA Polymerase is a recombinant-version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.. For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg 2+).A convenient 2X PCR master mix of Takara … hot start flight simulationWeb摘要：2024年5月，江苏扬州某鸭场25日龄左右樱桃谷鸭发生了以瘫痪为主要表现的神经症状疾病，病死鸭剖检无特征性病变。RT-PCR检测结果显示为鸭坦布苏病毒（T,现代畜牧cvonet,中国畜牧,特种养殖,肉食品,猪价,种猪,仔猪,生猪,西门塔尔,波尔山羊,鸡蛋,鸭蛋,饲料,玉米,兽药,养殖设备,孵化器,行情,标准 ... line-height 1.5 150%WebEasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous … line heat mapsWebSep 16, 2024 · PCR reaction system (25 μL) included 2.5 μL of 10 × EasyTaq ® Buffer, 2 μL of 2.5 mM dNTPs, 0.5 μL of 5 U/μL EasyTaq DNA Polymerase, 0.5 μL of 10 μM each primer pair of eight species, DNA mixture of eight species at the indicated concentration and refilled ddH 2 O to 25 μL. PCR amplification was performed by T100™ Thermal Cycler ... hotstar th activatehttp://cvonet.com/breed/detail/625798.html line-height 1.5什么意思WebOct 26, 2024 · A rapid, convenient, low-cost, and selective DNA isolation method was developed for identifying meat adulteration. A mesoporous metal organic framework (Meso-UIO-66)-coated solid phase microextraction system was employed as an isolation device to simplify DNA isolation into three steps (lysis, washing, and elution). Meso-UIO-66 was … line-height:1.4Web- EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase. - Extension rate is about 0.5 kb/min. ... DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4 Unit Definition One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable … line-height 1.4