Mouse backcrossing

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August undefined, 2024

Nettet11. apr. 2024 · Backcrossing is a two-generation breeding protocol that starts by generating hybrid F1 mice between two inbred strains (preferably distantly related), one of them carrying the mutation of interest. Then, F1 mice are mated with a member of one of the parental inbred strains to generate N2 mice. What does it mean to backcross mice? Nettet24. mai 2024 · Speed congenics is an important tool for creating congenic mice to investigate gene functions, but current SNP genotyping methods for speed congenics are expensive. These methods usually rely on chip or array technologies, and a different assay must be developed for each backcross strain combination. “Next generation” high …

Backcross breeding - PubMed

Nettet2 dager siden · Here we profiled and analysed genome-wide, ageing-related changes in transcriptional processes across different organisms: nematodes, fruitflies, mice, rats and humans. The average transcriptional ... NettetBackcross breeding enables breeders to transfer a desired trait such as a transgene from one variety (donor parent, DP) into the favored genetic background of another (recurrent parent, RP). If the trait of interest is produced by a dominant gene, this process involves four rounds of backcrossing wi … posti avoimet työpaikat oulu

How to Refresh Your Mutant or Transgenic Mouse Strains

NettetTraditional backcrossing is achieved by 10 successive backcrosses, which results in a new mouse model that is 99.9% identical to the recipient inbred strain. Speed Congenics or Accelerated Backcrossing Read the Taconic White Paper: Speed Congenics or Accelerated Backcrossing Nettet29. jun. 2012 · As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can either be back-crossed … NettetBackcross your strain ~10 times to your desired genetic background. A Genome Scan might help improve the speed at which this can be done, but may be limited in … posti harapainen lappeenranta

Mouse backcrossing

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NettetMice with chemically induced mutations are produced by using a variety of chemicals. One popular chemical mutagen, ethylnitrosourea (ENU), is used to induce point mutations. ENU mutagenesis involves exposing male mice to ENU and then mating the treated males to untreated females. Nettet18. aug. 2016 · Five generations of backcrossing will produce a line ~94% derived from the recipient genome. Five generations is the minimum number of crosses to …

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NettetBackcrossing is highly recommended to eliminate or reduce off-target mutations. Founder mice can be mosaic (having different mutations in different tissues or cells) and they may not transmit the mutation found in screening. Assistance in screening and breeding strategies can be provided upon request. Nettet7. des. 2016 · Speed backcrossing incorporates the analysis of single nucleotide polymorphisms (SNPs) distinguishing inbred mouse strains. Selecting progeny …

NettetWe demonstrated that the assay has a high density of diagnostic SNPs for backcrossing the BALB/c strain into the C57BL/6J strain (807–819 SNPs), and a sufficient density of diagnostic SNPs for ... NettetNational Center for Biotechnology Information

NettetCross mice to a genetically controlled inbred strain. At commercial suppliers or in controlled breeding colonies, inbred mice are rederived from archived stock every five … NettetBackcrossing is a crossing of a hybrid with one of its parents or an individual genetically similar to its parent, to achieve offspring with a genetic identity closer …

NettetBackcrossing is a two-generation breeding protocol that starts by generating hybrid F1 mice between two inbred strains (preferably distantly related), one of them carrying the …

NettetWhen establishing a closed breeding colony When new strains are received from collaborators During backcrossing (to check extent and/or for speed congenics) If unusual phenotypes appear At the commencement of a series of experiments Annual monitoring of in-house colonies No related content found. postihistoriallinen yhdistysNettet7. des. 2016 · Because ovulation can be induced in female mice as early as 3 weeks of age, superovulation-aided backcrossing of marker-selected females could … hann musichttp://www.informatics.jax.org/silver/chapters/3-2.shtml hanno city japanNettetDesired genotype achieved in fewer than 5 generations Custom-designed mouse 384 SNP panel for all donor and recipient strains Genome-wide microsatellite panels for rats, mice, and zebrafish All autosomes and the X chromosome covered in all panels Y-chromosome markers tested if needed Time Savings hannoa klaineanaNettetto phenotypic changes for mutant and transgenic mouse models. The way to mitigate the impact of genetic drift on mutant and transgenic mouse strains is to refresh the genetic … hannnannunipaNettetBackcrossing mice for 10 generations to produce heterozygous N10 mice and then intercrossing these mice to produce homozygous mice is traditionally the way of developing a congenic strain. This typically takes 2-3 years to complete. Using a marker assisted/speed congenics screening approach this timeframe can be halved. hannohNettet7. des. 2016 · The main difference between traditional and speed backcrossing described here is the use of superovulation, which has several implications including potential effects on genomic imprinting and practicability in specific mouse strains. Because speed backcrossing through the female germline relies on the same approach for marker … hanno 18 lustri